Social Context Restructures Behavioral Syntax in Mice

Abstract The study of social behavior in mice has grown increasingly relevant for unraveling associated brain circuits and advancing the development of treatments for psychiatric symptoms involving social withdrawal or social anxiety. However, a data-driven understanding of behavior and its modulation in solitary and social contexts is lacking. In this study, we employed motion sequencing (“MoSeq”) to decompose mouse behaviors into discrete units (“Syllables”) and investigate whether—and how—the behavioral repertoire differs between solitary and dyadic (social) settings. Our results reveal that social context significantly modulates a minority (25%) of syllables, containing predominantly stationary and undirected behaviors. Notably, these changes are associated with spatial proximity to another mouse rather than active social contact. Interestingly, a network analysis of syllable transitions shows that context-sensitive syllables exhibit altered network influence, independent of the number of connected syllables, suggesting a regulatory role. Furthermore, syllable composition changes significantly during social contact events with two distinct sequence families governing approach and withdrawal behaviors. However, no unique syllable sequences mapped to specific social interactions. Overall, our findings suggest that a subset of syllables drives contextual behavioral adaptation in mice, potentially fascilitating transitions within the broader behavioral repertoire. This highlights the utility of MoSeq in dissecting nuanced, context-dependent behavioral dynamics. Related files The preprint can be found at https://www.biorxiv.org/content/10.1101/2025.04.17.648924. The code used to generate the figures shown in the preprint based on this data can be found at Marti-Ritter/social-context-restructures-behavioral-syntax-in-mice. This data needs to be downloaded and all subdirectories unpacked, then the root_dir variable at the top of each notebook needs to be set to the path of the data directory.