Downregulation of MED12 via siRNA in prostate cancer cell lines (3’ tag DGE)

The Mediator complex is a multi-subunit protein that modulates gene expression on a genome-wide scale. MED12 and cyclin-dependent kinase 8 (CDK8) or its paralog CDK19 are components of its kinase module that regulate the proliferation of prostate cancer cells. In this study, we investigated how MED12 and CDK8/19 influence cancer-driven processes in prostate cancer cell lines, focusing on AR activity and enzalutamide resistance. To investigate the relevance of MED12 in enzalutamide sensitive and resistant prostate cancer cells, we performed siRNA mediated knockdown and analyzed the response using high-throughput 3’ tag differential gene expression analysis.