The proteomic and transcriptomic profiles of gastric mucosa from health volunteers

As the important layer of the stomach wall, gastric mucosa is related to many functions of digestion. According to the anatomical location, the human stomach is divided into seven regions. In order to get an entire profiling of the 7 regions at proteomic and transcriptomic levels, we collected 7 biopsies from each health volunteer, 49 total were used for integrative analysis, and measured the regional-specific gene products to define the reference intervals of gene expression for outliers screening and prognostic gene detection. Collectively, we presented profiling of normal gastric mucosa at different levels and defined reference ranges of gene expression to study the specific characteristics of abnormal and cancer samples, providing a better understanding of stomach physiological function in studies of gastric disease mechanism. Overall design: Following the histoanatomy of human stomach in medicine, we collected the gastric mucosa biopsies from the health volunteers without H. pylori infection. To keep the omic data at different expression levels have a consistent location for further comparison, after tissue homogenization, the powder was split into two parts for protein and mRNA extraction. Finally, the transcriptomic data was generated by RNAseq.