Single-cell RNA sequencing (scRNA-seq) on Treg cells from PBS and STAg treated mice

To identify cell composition and characterize transcriptional regulation in regulatory T (Treg) cells derived from mice subjected to soluble tachyzoite antigen (STAg) of Toxoplasma gondii treatment, we prepared splenic CD4+CD25+ Treg cells from PBS and STAg treated mice, and performed scRNA-seq using the 10× Genomics method. Using the 10× Genomics to analyze single-cell transcriptome in 2 types of Tregs pooled from PBS and STAg treated mice.