H3K27me3 demethylases maintain the transcriptional and epigenomic landscape of the intestinal epithelium [CHIP-seq]

To determine the contribution of H3K27me3 demethylases to intestinal homeostasis, an inducible mouse model was used to simultaneously ablate Kdm6a and Kdm6b expression from the intestinal epithelium. We then performed RNA-Seq gene expression analysis at two timepoints (7 days and 5 months) following induction of Kdm6a and Kdm6b ablation. Differential H3K27me3 regions were also identified using ChIP-Seq at one time point (7 days) following gene ablation. Chromatin-immunoprecipitation followed by sequencing (ChIP-Seq) for H3K27me3 in crypt intestinal epithelial cells collected from control and "double knock out" ("DKO") samples seven days after induction of Kdm6a and Kdm6b ablation