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Stress specific SCE1-interactome characterised by IP-MS analysis

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https://www.omicsdi.org/dataset/pride/PXD063447
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The AtSCE1-mVenus was immunoprecipitated using anti-GFP magnetic beads from Arbidopsis root cultures upon control, salt, mannitol and flg22 treatments. As a negative control, root tissues from pMDS1-VAM3 and pMDS1-SHR were utilized to account for non-specific binding to GFP. The eluate sapmles were digested using trypsin/Lys-C in gel and further LC-MS/MS analysis was performed using Thermo Fisher Q Exactive Mass Spectrometer.
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2025-09-08
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