We report the mutation profile of measles F gene by saturating mutagenesis. Overall design: We evaluate the mutation rate of measles F gene in pCDNA3 backbone (shuttle plasmid), pEMC backbone (genome
All primers were designed using PrimerQuest Tool (IDT) and purchased from IDT. Lyophilized primers were resuspended in DNAse/RNAse free water (Sigma-Aldrich) at a stock concentration of 1 μgr/μl, a fu
