The potassium channel subunit Kv1.8 (Kcna10) is essential for the distinctive outwardly rectifying conductances of type I and II vestibular hair cells

In amniotes, head motions and tilt are detected by two types of vestibular hair cells (HCs) with strikingly different morphology and physiology. Mature type I HCs express a large and very unusual potassium conductance, gK,L, which activates negative to resting potential, confers very negative resting potentials and low input resistances, and enhances an unusual non-quantal transmission from type I cells onto their calyceal afferent terminals. Following clues pointing to KV1.8 (KCNA10) in the Shaker K channel family as a candidate gK,L subunit, we compared whole-cell voltage-dependent currents from utricular hair cells of KV1.8-null mice and littermate controls. We found that KV1.8 is necessary not just for gK,L but also for fast-inactivating and delayed rectifier currents in type II HCs, which activate positive to resting potential. The distinct properties of the three KV1.8-dependent conductances may reflect different mixing with other KV1 subunits, such as KV1.4 (KCNA4). In KV1.8-null..., We compared whole-cell voltage-activated K+ currents in type I and type II hair cells from homozygous knockout (KV1.8–/–) animals and their wildtype (KV1.8+/+) or heterozygote (KV1.8+/–) littermates. We immunolocalized KV1.8 subunits in the utricular epithelium and pharmacologically characterized the residual K+ currents of KV1.8–/– animals. We performed current clamp experiments to assess the impact of KV1.8-dependent currents on passive membrane properties., , # The potassium channel subunit KV1.8 (Kcna10) is essential for the distinctive outwardly rectifying conductances of type I and II vestibular hair cells

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This README file was generated on 2023-10-06 by Hannah R. Martin. It can be read with Markdown syntax.\
These datasets contain processed (not raw) data used in the analyses of patch clamp electrophysiology in semi-intact utricles from mice.\
The study investigates the role of Kv1.8 conductances in vestibular hair cells.

### Description of the data and file structure

Datasets are in excel or csv format. Datasets have headers, and additional information is provided in this document.

#### GENERAL INFORMATION

1. Title of Dataset: The potassium channel subunit KV1.8 (Kcna10) is essential for the distinctive outwardly rectifying conductances of type I and II vestibular hair cells

2. Author Information
A. Principal Investigator Contact Information
Name: Ruth Anne Eatock
Institution: The University of Chicago
Addres...