Human intranasal olfactory biopsy analysis of Alzheimer's Disease subjects

Single-cell RNA sequencing was performed on brush biopsy samples from the human olfactory epithelium (n = 22 donors) spanning control (n = 6), pre-clinical (n = 9) and clinical (n = 7) Alzheimer's disease stages. The dataset comprises ~200,000 high-quality live cells encompassing neuronal, epithelial and immune lineages. These profiles enable comparative analyses of early neuroimmune changes associated with Alzheimer's progression and provide a resource for identifying peripheral biomarkers of disease. Overall design: Single cell 3' and 5' RNAseq of live olfactory epithelium from 22 adult human Control (n=6), Pre-Clinical Alzheimer's (n=9) or Clinical Alzheimer's (n=7) (based on cognitive function and CSF Aß42/Aß40 ratio) using the 10X Genomics Chromium platform and sequenced on Illumina NovaSeqX. Control Subject 20, Clinical AD Subject 21, and Clinical AD Subject 22 were 5' scRNAseq, rest are 3' scRNAseq.