The effect of new probiotic mixture in DSS (dextran sulphate sodium) – induced colitis mouse model

The current study was designed to clarify signalling pathways and assess possible beneficial effect of new probiotic mixture in DSS (dextran sulphate sodium) – induced colitis mouse model. Manipulation of intestinal microbiota with probiotics represents a promising alternative or adjunct therapy in gastrointestinal disorders and inflammation. RNA extracted from the middle part of colon tissue from ~11 weeks female C57BL/6JOlaHsd mouse strain was used for examination of the global gene expression using Affymetrix GeneChip Mouse Gene 2.0 ST microarrays. Two factor experimental design: two conditions (Healthy and DSS-induced colitis) and three treatments (None-saline, Probiotic, CsA-cyclosporine A), w/o CsA treatment of healthy animals. 4 biological replicates per condition, 20 mice altogether.