Basal-to-inflammatory transition enhances basal cell carcinoma (BCC) therapy resistance via crosstalk with a pro-inflammatory stromal niche [RNA-seq II]

The goal of these RNA-seq experiments was to determine transcriptomic gene expression changes caused by 7 days of Il1a and Osm combination treatment of mouse primary BCC tumor-derived organoids compared to PBS control Mouse primary BCC tumor tissues were embedded as small pieces in basement membrane extract and grown in organoid medium for 7 days. Cells were treated with either PBS control or Il1 and Osm ligand combination for 7 days in organoid medium.