The increase of miR-378a-3p in keratinocyte associated with pathogenesis of psoriasis

We report the high-throughput profiling of human primary keratinocytes transfected with either miR-378 mimic or miR-378 inhibitor at 24 or 48 hours. Our study focused on the dysregulation of microRNAs that might partially be a fundamental mechanism leading to psoriasis development. We knock-in and knock-out a specific miR-378 and see the cellular responses. As expected, overexpression of miR-378a inhibited the proliferation, enhanced the apoptosis and disturbed the cell cycle. Regarding to the confirm experiment, our results suggested that the expression of miR-378a associated with the psoriasis pathogenesis enhancing the severity of psoriatic lesion, and might useful for further development of therapeutic strategy for psoriasis. Overall design: We investigated microRNA expression in psoriatic skin. Experiment found miR-378 was dsyregulated in the skin biopsy from patients. To investigate the entire effects of miR-378, overall design was eventually come out with RNA-sequencing on 4 groups at 2 timepoints (24 and 48 hours). These are including miR-378 mimic transfection, control mimic transfection, miR-378 inhibitor transfection, and control inhibitor transfection.