sciMETv3 for atlas-scale single-cell DNA methylation analysis

We describe sciMETv3, a combinatorial indexing-based technique that is capable of producing single-cell DNA methylation datasets with 100's of thousands of cells in a single experiment by one individual over two days. sciMETv3 is compatible with capture techniques to reduce sequencing costs (sciMET-cap) as well as enzymatic conversion methods. sciMETv3 libraries can be designed for compatibility with both Illumina and Ultima sequencing platforms. Four speciems were used in this study, each a BA46 cortex specimen from a healthy donor and processed using sciMETv3.