Volcani-DD-1 WGS vs IWGSC RefSeq v1.0 Genome Assembly

WGS reads of Volcani-DD-1 (http://dx.doi.org/10.1111/j.1467-7652.2012.00717.x) were aligned against the IWGSC Triticum aestivum Chinese Spring RefSeq v1.0 genome assembly using Minimap2. These BAM files (.bam) and index files (.bam.bai) are provided together with a summary of read alignment coverage bigWig files (.bam.bw). SNP variants were called from these BAM files to generate VCF files (.bam.vcf.gz) and index files (.bam.vcf.gz.tbi) and are provided together with a summary of SNP density (SNPs per 10 kbp) bigWig files (.bam.vcf.w10000_s10000.bw). VCF files contain the following filter values and corresponding meaning: PASS = high quality (Q>=30) homozygous; Het = high quality (Q>=30) heterozygous; LowQualHom = low quality (Q

Volcani-DD-1 的全基因组序列数据（http://dx.doi.org/10.1111/j.1467-7652.2012.00717.x）经过 Minimap2 软件与 IWGSC 提供的小麦（Triticum aestivum）中国春参考基因组序列（v1.0）进行比对。所生成的 BAM 文件（.bam）及其索引文件（.bam.bai）以及读取比对覆盖率的 bigWig 文件（.bam.bw）一同提供。通过这些 BAM 文件调用 SNP 变异，生成 VCF 文件（.bam.vcf.gz）及其索引文件（.bam.vcf.gz.tbi），并附有 SNP 密度（每 10 kbp 中的 SNP 数量）的 bigWig 文件（.bam.vcf.w10000_s10000.bw）。VCF 文件中包含以下过滤值及其对应含义：PASS = 高质量（Q值≥30）的纯合子；Het = 高质量（Q值≥30）的杂合子；LowQualHom = 低质量（Q值<30）的纯合子。