five

SA-AgNP and CD25

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NIAID Data Ecosystem2026-03-11 收录
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http://flowrepository.org/id/FR-FCM-ZZRX
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Evaluate and compare indirect detection of CD25 on T cells by SA-AgNP in mass cytometry Conclusion: The titration revealed an optimal concentration of 40 nm SA-AgNP for detection of CD25-bio of 1:25. Signal intensities achieved by 40 nm SA-AgNP on CD25-bio were higher than direct detection by CD25-149Sm or indirect detection by anti-bio-165Ho. This allowed not only a distinct and specific discrimination of CD25hiCD127− regulatory T cells (Treg), but also improved the separation of CD25loCD127+ cells from the CD25–CD127lo population within CD4+ T cells and enabled the discrimination of CD25-expressing B cells Notes: see Fig. 3; Fig. S5 no primary antibody controls comparison to direct detection (CD25-149Sm) or indirect detection (anti-bio-165Ho)
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2019-03-01
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