Species specific metabolic capacity of naphthalene and phenanthrene in three species of marine teleosts exposed to Deepwater Horizon Crude oil

Select monohydroxylated polycyclic aromatic hydrocarbons (PAHs) metabolites were measured in three marine teleosts, red drum (Sciaenops ocellatus), Florida pompano (Trachinotus carolinus) and southern flounder (Paralichthys lethostigma) to evaluate species specific metabolic differences. Fish were exposed to DWH crude oil by intraperitoneal (i.p.) injections at time 0 and 48 hours with bile sampling events at 24 and 72 hours post injection. Biliary levels of monohydroxylated naphthalene and phenanthrene metabolites were confirmed and quantified using gas chromatography triple quadrapole mass spectrometry.本研究针对单羟基化多环芳烃（polycyclic aromatic hydrocarbons, PAHs）代谢物，在红鼓鱼（Sciaenops ocellatus）、佛罗里达仿鲳鲹（Trachinotus carolinus）及南方牙鲆（Paralichthys lethostigma）三种海洋硬骨鱼类中开展检测，以评估其物种特异性代谢差异。实验鱼于0小时与48小时通过腹腔（intraperitoneal, i.p.）注射暴露于DWH原油，并分别于注射后24小时和72小时采集胆汁样本。采用气相色谱-三重四极杆质谱法，对胆汁中单羟基化萘与菲代谢物的含量进行定性确证与定量分析。