Supplementary Movie 4. Invadopodia in RhoG KD SUM159 cells.

SUM159 cells stably expressing a RhoG targeting shRNA were infected with mCherry-cortactin adenovirus as in Supp Movie 1. At indicated times cells were stimulated to form invadopodia with PDBu. Images were captured at 15 sec intervals for the duration of the movie.将稳定表达靶向RhoG的短发夹RNA（short hairpin RNA, shRNA）的SUM159细胞，参照补充视频1中的操作步骤，用搭载mCherry-皮层肌动蛋白（cortactin）的腺病毒进行感染。随后在指定时间点，以PDBu（phorbol 12,13-二丁酸酯）诱导细胞形成侵袭伪足（invadopodia）。在整个成像时长内，以15秒的间隔采集图像。