E. coli Sacculi +A22 (MG1655)

Tilt Series Date: 2015-04-10 Data Taken By: Matt Swulius Species / Specimen: Escherichia coli Strain: MG1655 sacculi Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. Microscope: Caltech Polara Acquisition Software: UCSFTomo Upload Method: pipeline Processing Software Used: raptor Sample Preparation: Cells were treated for one hour with 10ug/ml A22 until they were rounded and lemon-shaped prior to sacculus purification.倾斜序列采集日期：2015-04-10 数据采集者：马特·斯威利乌斯（Matt Swulius） 物种/样本：大肠杆菌（Escherichia coli） 菌株：MG1655 囊体（sacculi） 倾斜序列采集参数：单轴倾斜，倾斜范围为(-60.0°, 60.0°)，步进角度1°，采用恒定角增量，电子剂量150.0 eV/Ų，欠焦值-6.0 μm，放大倍数22500倍 显微镜：Caltech Polara 采集软件：UCSFTomo 上传方式：流水线式上传 所用处理软件：raptor 样本制备：在进行囊体纯化前，将细胞以10μg/mL的A22处理1小时，直至细胞变为圆形或柠檬状