Enhancing comparative T-cell receptor repertoire analysis in small biological samples through pooling homologous cell samples from multiple mice

Amplification and sequencing techniques for bulk-cell T-cell receptor (TCR) sequencing have improved in the last decade, allowing in depth characterization of the TCR repertoire with high accuracy. Nevertheless, tackling the TCR repertoire diverse and complex nature remains challenging for in small samples. Herein, we address this problematic by pooling homologous biological samples to collect enough input material and evaluating the effect of pooling on through a set of TCR repertoire statistics.