Transcriptional reprogramming of cancer cells upon loss of the PPM1G/PP2Cγ phosphatase

Dysfunction of cell signaling–transcriptional regulatory circuits promotes malignant transformation, tumor development, and resistance to cancer therapy. However, the molecular mechanisms by which these abnormal programs translate into tumorigenesis are not entirely understood. The PPM1G/PP2Cγ phosphatase is frequently mutated and deleted in cancer but the functional consequences remain unknown. Here we measure steady-state and nascent RNA changes in parental and PPM1G knockout colorectal cancer cells, and define the transcriptional signatures deregulated upon PPM1G loss leading to aberrant, increased cell proliferation indicating PPM1G is a novel tumor suppressor. Nascent and steady-state RNA expression levels in Ctrl vs PPM1G KO cells.