Additional file 3 of Dimethyl fumarate reduces TNF and Plasmodium falciparum induced brain endothelium activation in vitro
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Additional file 3. Differentially expressed transcripts in DMF pretreated TNF treated HBMVEC compared to TNF treatment. HBMVEC were preincubated with DMF (50 μM) for 1 h prior to the addition of TNF incubation (10 ng/ml) or TNF incubation alone for 6 h. Total RNA was extracted, reverse-transcribed, amplified and hybridized to a microarray. Gene expression profiles were generated using a robust multi-array average algorithm followed by quantile normalization and batch correction. Data represents three independent experiments, each done in triplicate. 348 transcripts were significantly differentially abundant between experimental groups using a Student’s t test and FDR (fold change > 2, FDR ≤ 0.01, p
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figshare
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2020-10-22
