CRISPR/Cas9-based Mutations in DND1 Gene Enhance Tomato Resistance to Powdery Mildew with Low Fitness Costs

This research uses CRISPR-Cas9 technology to target the DND1 gene in tomatoes to enhance resistance to powdery mildew. The study focused on three T1 lines (E1, E3, E4) and highlighted the promising results of the E3 line, which showed reduced disease symptoms and minimal fitness costs. The research suggests the potential for breeding disease-resistant tomatoes using dnd1 mutants.