Single-cell RNA-seq of goat early embyos and GFFs cells

We use high-throughput single cell RNA-seq to determine expression profile of IVF-2cell 、 IVF-8cell and SCNT-8cell embryos,and C1 and over3720. At present, the strand-specific library could not be generated for the analysis of goat embryo lncRNA because of the lack of available specific rRNA removal kits for goats. In this study, single-cell RNA-seq technology was used to analyze early embryonic transcripts, enrich the involving transcripts, and construct an common transcript library. For identifying transcripts expressed during the early goat embryonic stage, three cDNA libraries were established from early goat embryos at the three developmental stages, namely, two-cell (IVF-2c)、 eight-cell embryos (IVF-8c) and somatic cell nuclear transfer（SCNT-8c). The GFFs cells transfected with control (no-load) and recombinant vectors were collected, total RNA was extracted, and the quality of the RNA was determined. Examination of expression differences between goat IVF 2-cell 、IVF 8-cell emryos and SCNT 8-cell