The effect of lncRNA PART1 knockdown on mature miRNA levels in HCC1806 and HCC1395 cells

PART1 was knocked down down using GapmeRs specific to PART1. The breast cancer cells used in this study were confirmed to have PART1 expression by qPCR and knockdown of PART was confirmed by qPCR. To assess the changes to mature miRNAs post PART1 knockdown, miRNA 4.0 microarray analyses were completed on HCC1806 and HCC1395 cells were treated with either control GapmeR, GapmeR#1 or GapmeR #2 for 48 hours and then collected and RNA purified using the mirVana™ miRNA Isolation Kit. Samples (in biological triplicate; n=3) were sent to the Microarray Facility, The Centre for Applied Genomics (TCAG), The Hospital for Sick Children (Toronto, Canada) where the processed for Affymetrix miRNA 4.0 microarray platform, and miRNA differences quantified. Data was collected in raw CEL format and processed through the Transcriptome Analysis Console (Affymetrix) to reveal differential miRNA levels.