Identifying crossover locations in Arabidopsis thaliana h2a.w7 Col-0 × Ler F2 populations using genotyping-by-sequencing

Genomic DNA from 144 h2a.w7 Col-0 x Ler F2 individuals was extracted using CTAB (as described in Kuo et al, 2022 Methods Mol Biol - PMID: 35461443) and used to generate sequencing libraries as described (Lawrence et al, 2019 Current Biology - PMID: 31378616). Sequencing data was analysed to identify crossovers using the TIGER pipeline as previously described (Rowan et al, 2015 G3 (Bethesda) - PMID: 25585881; Yelina et al, 2015 Genes & Dev - PMID: 26494791; Lawrence et al, 2019 Current Biology - PMID: 31378616).