Time point RNA-seq of Dux over expression samples reveals different transcriptomes of Dux induction in mESC

Prevous study has discoverd 2CLC trancriptome and MERVL can be up-regulated by Dux over expression,but rarely has been found about the whole procedure of Dux-induced 2C-entry and exit process and whether this system can reflect the natural 2-cell embryo development,so we we defined different time point transcription after Dux over expression,and try to establish a link between gene expression profile between gene dynamics in different Dux over expression time point and in early mouse embryo development Overall design: We then performed gene expression clustering analysis using data obtained from RNA-seq of 0h,12h,24h,36h,48h,72h samples after Dux over expression