In this study, we have uploaded the transcriptome sequencing dataset of GL3 leaf explants during regeneration on callus induction medium to the NCBI database. The detailed experimental treatments and culture conditions are as follows: GL3 leaves were

In this study, we have uploaded the transcriptome sequencing dataset of GL3 leaf explants during regeneration on callus induction medium to the NCBI database.The detailed experimental treatments and culture conditions are as follows: GL3 leaves were used as experimental materials. The GL3 leaves were processed into explants by longitudinally cutting perpendicular to the leaf veins, removing leaf tips and petioles, and retaining leaf segments with incisions at both ends. These leaf segment explants were inoculated onto the callus induction medium, cultured in the dark for 0, 1, 3, 5, 9, 14, 21, and 32 days respectively, and the harvested materials at each time point were subjected to transcriptome sequencing.This dataset documents the transcript expression profiles of GL3 leaf explants at distinct time points (including 0, 1, 3, 5, 9, 14, 21, 32 days) during regeneration on callus induction medium. It enables dissection of the dynamic molecular regulatory network governing callus induction and regeneration processes in GL3 leaf explants, facilitates identification of key genes associated with these sequential developmental events, and provides foundational transcriptomic data for investigating the molecular mechanisms of plant tissue culture regeneration. The dataset has been uploaded to the NCBI database, and is available for query and in-depth secondary analysis by researchers in the fields of plant molecular biology and plant tissue culture.