Vitis vinifera Transcriptome or Gene expression. Vitis vinifera

Changes in gene expression during berry development during a grape growing season were analysed. The effect on gene expression of different viticultural practises during grape berry development was investigated in this study by comparing two pruning methods (spur versus machine). Grape berries were collected and pooled on a weekly basis to obtain a developmental series comprised of 17 developmental stages from flowering until harvest across the grape growing season for both spur and machine pruned vines. Gene expression patterns during development and between pruning treatments were obtained. Keywords: Time course, developmental series and treatments Overall design: Seventeen consecutive developmental stages were analyzed for gene expression changes using the Affymetrix one colour array platform. The developmental stages were numbered 1 to 17. Two biological replicates were collected for each developmental stage. Biotin labeled probes were prepared from the extracted RNA from each of the pooled berry samples and hybridized to the Vitis vinifera GeneChip. The sample/GeneChip position on the Affymetrix hybridization station was randomized using the AlgDesign package from R.