Maternal hypoxia exposure perturbs the methylation of imprinted genes in adult sperm and causes intergenerational placental impairments in male offspring

Hypobaric hypoxia at high altitudes adversely affects the health and fertility of humans and animals globally.The results revealed that maternal hypoxia mildly affected sperm DNA methylation in F1 males and caused severe developmental defects in F2 offspring in a male-biased manner. Notably, after mating F1 males with control females, a significant number of the male F2 fetuses were lost at embryonic day (E) 13.5 because of placental defects. Integrated analyses of RNA-seq and genome-wide DNA methylation (WGBS) data revealed that placentae from these male fetuses aberrantly expressed a list of imprinted genes, including Gnas, Slc38a4, Jade1 and Kcnq1 which are also differentially methylated in F1 sperm. In this dataset, we upload these rowdata as followin "ZR2025" 1 RNASEQ included the RNAseq rowdata by E13.5 male placenta the control named "CM1_1, CM1_2, CM2_1, CM2_2, CM3_1, CM2_2"the hypoxia group named "HM1_1, HM1_2, HM2_1, HM2_2, HM3_1, HM2_2"2 "WGBS" included the WGBS rowdata by E13.5 male placenta (1) placenta: the control named "placenta-CM1_1, CM2_1, CM2_2," included in "placenta" folder(2)WGBS: the control named "placenta-CM1_2, " and "the hypoxia group named "placenta-HM1_1, HM1_2, HM2_1, HM2_2," included in "WGBS" folder