Genetic dependencies reflect functional relationships.

(A) Genetic interaction patterns between secretory and cell division factors. (B) Growth rates (fitness) of secY and ftsK single and double mutants in liquid rich (RM) or minimal medium (MM) at 32°C over 24 h; hypomorphic alleles indicated by asterisks. (C) Pair-wise correlation coefficient profiles of secretory and cell division mutants grown on rich medium or minimal medium. (D) Identification of Sec proteins co-purified from the endogenous affinity tagged Fts protein by tandem mass spectrometry. (E) Fluorescent microscopy of E. coli stains (stained with FM4-64) after growth in minimal or rich medium; scale-bars 2 µm. (F) Quantitative measurements of changes in mutant cell length after growth in rich and minimal medium. Error bars show measurement of standard deviation from three independent experiments.