Transcriptome analysis of the CBX3-depleted 143B osteosarcoma cell line.. Transcriptome analysis of the CBX3-depleted 143B osteosarcoma cell line.

To investigate the role of CBX3 in the progression of osteosarcoma, we performed a transcriptome analysis of the CBX3-depleted 143B cell line and a matched normal 143B cell line.Differential gene enrichment revealed activation of the anti-DNA damage pathway and activation of the apoptotic and P53 pathways. Overall design: The mRNA profiles of 143B osteosarcoma cells without targeting gene small interfering RNA and targeting CBX3 small molecule interfering RNA were generated by deep sequencing using a Novaseq 6000 sequencer (Illumina).