RBPMS mediates smooth muscle specific alternative splicing via oligomerization and modulation of trans factor recruitment

RBPMS regulate splicing decisions by modulating the transcript-bound proteome. This experiment aims to investigate the proteome assembled on an experimental RNA, TM3 transcript, in human Hela nuclear extract under the various conditions, ± ATP and ± RBPMS. The model RNA is tethered on amylose bead via a protein, MPB-MS2. After pull-down, the protein-RNA complexes are eluted from the beads with maltose. The sample series "N" and "NA" are two negative control conditions. "B" and "R" are sample series of two experimental conditions.