RNA-sequencing assay to identify changed gene expression in lung adenocarcinoma (LUAD) cells before and after osimertinib resistance

The H1975 osimertinib resistant (H1975 OR) cell lines were successfully established by subjecting parental H1975 cells to a gradually increasing concentration of osimertinib up to 1μM for a duration exceeding 2 months. In order to investigate the molecular alterations associated with osimertinib resistance, we analyzed the transcriptional profiling of parental H1975 cells and H1975 OR cells by RNA sequencing. Three biological replicates for each group H1975 and H1975 OR were subjected to RNA-sequencing analysis respectively, among which H1975 acted as the reference sample for H1975 OR.