Single-nuclei sequencing of mouse brain tissue and single-cell sequencing of FACS-sorted mouse astrocytes, investigating the effect of absence of microglia in the developing brain

Microglia, brain-resident macrophages, have been proposed to play an active role in synaptic refinement and maturation, influencing plasticity and circuit-level connectivity. Using a genetically modified mouse which lacks microglia (Csf1r deltaFIRE/deltaFIRE), we investigate the effect on gene expression in particular cell types of the presence or absence of microglia in the developing mouse brain.