Tissue-specific reprogramming of host tRNA transcriptome by the microbiome

The role of gut microbiota in modulating host tRNA modifications and expression has not yet been studied. In this experiment, we applied DM-tRNA-seq and normal tRNA-seq to obtain and infer the cytosolic and mitochondrial tRNA modifications and expression of four tissues (brain, intestine, kidney, and liver) in the specific pathogen-free (SPF) and germ-free (GF) mice, respectively. In conclusion, we confirmed that the modifications and expression of both cytosolic and mitochondrial tRNAs were influenced by not only tissue-specific but also microbiota-dependent manners. The present study facilitates comprehensive insights and better understanding into the interactions between the gut microbiota and the tRNA modifications and expression among host tissues Overall design: We apply the DM-tRNA-seq and normal tRNA-seq to a total of 48 samples (SPF/GF mice, four tissues (brain, intestine, kidney, and liver), demethylase-treated/untreated, and three replicates). tRNA modifications and expression of their sequencing data are further analyzed in this study