Extracted Chlorophyll and Phaeopigment data collected from R/V Endeavor cruises EN259, EN262, EN264, EN266, and EN267II in the Gulf of Maine and Georges Bank in 1995 as part of the U.S. GLOBEC program (GB project)
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<h2>Extracted Chlorophyll and Phaeopigment</h2> <p><strong>DMO note:</strong> The data reported consists of three replicates per each depth horizon sampled.</p> <p><strong>PI Responsible: Dian J. Gifford</strong></p> <p>Samples for water column chlorophyll and phaeopigment were collected and analyzed during the following Vital rates 1995 U.S. GLOBEC Georges Bank process cruises:</p> <p> </p> <p> </p> <ul> <li>EN259</li> <li>EN262</li> <li>EN264</li> <li>EN266</li> <li>EN267B (aka EN267II and EN267 LEG 2) <p>Water Collection: Seawater was collected using 10-L teflon-lined Go-flo bottles mounted on the Neil Brown CTD rosette. Water was drained into opaque brown 1-L bottles immediately after collection and refrigerated until processed. Samples were collected onto filters within one hour of water collection. In areas where the water column was well mixed, water was collected from the top, middle and bottom of the water column. When the water column was stratified, water was collected from the top, middles and bottom of the water column as well as around the hydrographic features of interest.</p> <p>Sample Processing: Samples were prepared for total, <20 µm, and <5 µm chlorophyll and phaeopigment. Samples for total pigments consisted of bulk seawater. Samples for < 20 µm and < 5 µm pigments were passed gently through clean Nitex meshes of appropriate porosity and the filtrate retained for analysis. Three replicate 50-ml samples of each size fraction were collected onto 25 mm GF/F filters, placed into 5 ml of 90% acetone in a capped test tube, and extracted in the freezer for 24 hours prior to analysis.</p> <p>Sample Analysis: The filters were removed from defrosted test tubes with a clean stainless steel spatula, the tube wiped clean with a Kimwipe, and samples read on a Turner Designs Model 10 fluorometer before and after acidification with 10% HCl (Parsons et al., 1984).</p> <p>Caveat: In general, pigment concentrations in the <5 µm samples were approximately equal to the <20 µm samples (i.e., there was very little chlorophyll in the 5-20 µm size range: most chlorophyll < 20 µm was also < 5 µm). Because of the difficulty of passing seawater quantitatively through the 5 µm mesh, the <5 µm data are more variable than the Total and < 20 µm data. To avoid confusion, the < 5 µm data are not included in the data files. The data are available, and scientific investigators who need it should contact the PI directly.</p> <p>Data Use: The data are available for use by any scientific investigator who wishes to use them. The PI must be consulted prior to publication.</p> <pre> <strong>Data Submitted by:</strong> Dian J. Gifford Graduate School of Oceanography University of Rhode Island Narragansett, RI 02882-1197 voice: 401-874-6690 fax: 401-874-6240 e-mail: <a href=\"mailto:gifford@gsosun1.gso.uri.edu\">gifford@gsosun1.gso.uri.edu</a> <em>updated: Aug 05. 2005, gfh</em> </pre> </li> </ul> <p> </p> <p> </p>
## 提取态叶绿素与褐色素（Extracted Chlorophyll and Phaeopigment） **DMO备注：** 本次发布的数据为每个采样深度层的三次重复实验结果。 **首席研究员（Principal Investigator, PI）：** 戴安·J·吉福德（Dian J. Gifford） 本次水层叶绿素（chlorophyll）与褐色素（phaeopigment）样品的采集与分析工作，依托1995年美国全球海洋生态系统动力学计划（U.S. GLOBEC）乔治滩（Georges Bank）过程航次的"Vital rates"项目开展，涉及航次如下： - EN259 - EN262 - EN264 - EN266 - EN267B（又名EN267II及EN267 LEG 2） **采样流程：** 海水样品通过安装于Neil Brown型温盐深采水架（CTD rosette）上的10升特氟龙内衬Go-Flo采水器（Go-flo bottles）采集。采集后立即将海水转移至不透明棕色1升采样瓶中，冷藏保存直至处理。采样后1小时内将样品过滤至滤膜上。若水柱混合均匀，则分别采集水柱表层、中层及底层水样；若水柱存在层结，则除采集表层、中层及底层水样外，还会采集目标水文特征周边区域的水样。 **样品前处理：** 本次实验针对总组分、<20 µm粒径级及<5 µm粒径级的叶绿素与褐色素开展样品制备。总色素样品采用未经分级的全量海水制备；<20 µm及<5 µm粒径级的色素样品则通过轻柔过滤的方式，经孔径匹配的洁净Nitex筛网（Nitex meshes）过滤，收集滤液用于后续分析。每个粒径级均取三次重复的50 mL滤液，过滤至25 mm GF/F滤膜（GF/F filters）上；随后将滤膜置于装有5 mL 90%丙酮（acetone）的带盖试管中，于冷冻环境下萃取24小时后再进行分析。 **样品分析：** 使用洁净不锈钢刮刀将滤膜从解冻后的试管中取出，用Kimwipe无尘擦拭纸擦拭试管内壁，随后使用Turner Designs 10型荧光计（Turner Designs Model 10 fluorometer）在加入10%盐酸（HCl）酸化前后分别测定样品荧光值（Parsons等，1984）。 **数据说明：** 总体而言，<5 µm粒径级样品的色素浓度与<20 µm粒径级样品大致相当（即5-20 µm粒径范围内的叶绿素含量极低：绝大多数<20 µm的叶绿素同时也<5 µm）。由于难以将海水完全定量地通过5 µm筛网，<5 µm粒径级的数据相较于总样及<20 µm粒径级数据的波动性更大。为避免混淆，本次发布的数据文件中未包含<5 µm粒径级的数据集。该数据仍可获取，有需求的科研人员可直接联系首席研究员。 **数据使用规范：** 所有科研人员均可使用本数据集，但在发表相关研究成果前需征得首席研究员的同意。 **数据提交人：** 戴安·J·吉福德（Dian J. Gifford） 罗德岛大学海洋学研究生院（Graduate School of Oceanography, University of Rhode Island） 美国罗得岛州纳拉甘西特，邮编02882-1197 电话：401-874-6690 传真：401-874-6240 电子邮箱：<a href="mailto:gifford@gsosun1.gso.uri.edu">gifford@gsosun1.gso.uri.edu</a> <em>更新时间：2005年8月5日，gfh</em>
