Data underlying the publication: Clonal Amplification-Enhanced Gene Expression in Synthetic Vesicles

Data behind the publication: 1. Abil, Z., Restrepo Sierra, A. M. &amp; Danelon, C. Clonal Amplification-Enhanced Gene Expression in Synthetic Vesicles. ACS Synth. Biol. 12, 1187–1203 (2023).<br>The hereby provided research was designed and performed as part of the synthetic biology and BaSyc (NL) consortium efforts to build a synthetic cell from the bottom up. The dataset includes '.prism' files with the raw data utilized for constructing the figures from the above stated paper. Besides these files, the provided .zip files also contain raw data that includes both flow cytometry and confocal microscopy data. Each compressed file indicates to which figure(s) it should belong to and which type of data does it contain. Some folders (i.e., some of the ones with FACS data) also include a .MATLAB file with a small script connected to each file for easier visualization of the data. The confocal imaging data (if not in PNG) can be opened and analyzed with FIJI. The dataset also includes two separate MATLAB files labeled with 'SMELDit', two MATLAB scripts utilized for the analysis of confocal images from Fig. 6 of the above mentioned paper. The additional PDF labeled: MEP_thesis_Mats van Tongeren contains a more specific description and instructions on how to operate the program. Finally, the dataset also contains the SnapGene files with the different DNA sequences utilized on the experiments of the above mentioned paper.<br>If additional information is needed, please contact [e-mail] or [e-mail], or also the corresponding author of the paper: Christophe Danelon.

本数据集对应发表论文：1. Abil, Z.、Restrepo Sierra, A. M. 与 Danelon, C. 发表于《ACS合成生物学》（ACS Synth. Biol.）2023年第12卷第1187-1203页的论文《克隆扩增增强合成囊泡中的基因表达》（Clonal Amplification-Enhanced Gene Expression in Synthetic Vesicles）。
本次公开的研究系合成生物学与荷兰BaSyc联盟（BaSyc (NL)）为自下而上构建人工细胞所开展工作的一部分。本数据集包含用于复现上述论文各图表的原始数据.prism文件。除上述文件外，本次发布的.zip压缩包还包含流式细胞术与共聚焦显微镜成像的原始数据。每个压缩包均标注了其对应支撑的图表编号及所含数据类型。部分文件夹（即包含荧光激活细胞分选（FACS）数据的部分文件夹）还附带了针对各数据文件的.MATLAB脚本文件，用于简化数据可视化流程。非PNG格式的共聚焦成像数据可通过FIJI（斐济图像分析软件）打开并进行分析。本数据集还包含两个标注为"SMELDit"的MATLAB脚本文件，用于对上述论文图6的共聚焦图像进行分析。文件MEP_thesis_Mats van Tongeren（PDF格式）提供了该程序的详细说明与操作指南。最后，本数据集还包含用于上述论文各项实验的不同DNA序列的SnapGene文件。
若需获取更多相关信息，请联系邮箱[e-mail]或[e-mail]，亦可联系论文通讯作者Christophe Danelon。