Mauricio Valerio-Santiago, Fernando Monje-Casas (2011) CIL:13890, Saccharomyces cerevisiae. CIL. Dataset
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3HA-Bfa1 (red) is generally localized to one spindle pole body (SPB) in wild-type cells in metaphase as determined by spindle morphology (tubulin, green) and nuclear morphology (DAPI, blue). Control image for CIL# 13891 in which constitutive targeting of Tem1 to both SPBs leads to symmetric SPB localization of Bfa1. Image is Fig 4C, top panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 4 include CIL# 13890, 13891, 13892, 13893.
3HA-Bfa1(红色)在野生型细胞的有丝分裂中期通常定位于一个纺锤体极体(SPB)中,这一现象可通过纺锤体形态(微管,绿色)和核形态(DAPI,蓝色)进行确认。在CIL# 13891的控制图像中,由于Tem1的组成性靶向定位于两个SPB,导致Bfa1在SPB中的定位呈现对称性。图像为J Cell Biol.(2011)192: 599-614中的图4C,顶部面板。图4中包括CIL# 13890、13891、13892、13893的图像。
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