Expression data from plasma exosomal microRNA of rats with remote ischemic conditioning and normal controls

Remote ischemic conditioning (RIC) is a safely therapeutic strategy for a variety of ischemic diseases, with efficiency comparable to the classic in-situ ischemic methods. Recent evidence has shown that RIC could alter the content of circulating exosomes. microRNAs involves in numerous functionally different biological and physiological processes. The aim of this study is to identify the differentially expressed exosomal microRNAs in rat plasma with RIC and to further explore the functions in ischemic heart diseases. Five RIC rats and 5 control rats were enrolled in this study. We used microarray analysis to identify miRNA expression in their plasma exosomes. A total of 5 rats with RIC and 5 healthy controls participated in the study. Wistar rats were subjected to four cycles of remote ischemia, and each cycle consisted of 5-minute ischemia and 5-minute reperfusion. We collected venous blood (10 ml) from each participant using an EDTA-anticoagulated tube. Exosomes were isolated via differential centrifugation.