Hypoxia-Induced Metabolic Reprogramming and Markings of Cell Fate in Concentric Arterial Hypertrophy

Video S1: Ren1c-lineage cells are localized to the juxtaglomerular area under normal conditions, related to Figure 1. Three-dimensional visualization of Ren1c-lineage cells (GFP, green) and surrounding background structures (tdTomato, red) in the kidney cortex of a 1-month-old Ren1c+/-;Ren1cCre;R26RmTmG mouse. Video S2: Concentric arterial and arteriolar hypertrophy (CAAH) in Ren1cKO mouse, related to Figure 1. Three-dimensional distribution of Reninnull/Ren1cKO cells in 1-month-old Ren1c-/-;Ren1cCre;R26RmTmG mouse. Hypertrophied Reninnull cells accumulate in arterioles, forming CAAH that often encircle the glomeruli. Reninnull cells are also broadly distributed along arcuate arteries. Video S3: Three-dimensional morphology of the renal vascular tree and renin cells under normal conditions, related to Figure 1. Kidney cortex from a 5-month-old SMMHCCreERT2;R26RtdTomato;Ren1cYFP mouse (control). Vascular smooth muscle is labeled with tdTomato (red), and renin cells (YFP, yellow) are localized exclusively at the tips of afferent arterioles. Video S4: Concentric arterial and arteriolar hypertrophy (CAAH) in captopril-treated mouse, related to Figure 1. Kidney cortex from a 5-month-old SMMHCCreERT2;R26RtdTomato;Ren1cYFP mouse treated with captopril for 3 months. The tips of afferent arterioles are thickened in a goblet-like configuration and enveloped by renin cells, forming CAAH. Striated extensions (recruitment) of renin cells are frequently observed along the renal vascular tree (tdTomato, red). Metabolomics data: Urine metabolomics raw counts data for 1 and 6-month-old Ren1cKO and control mice, related to Figures 4 and 5. Raw spectral counts for control and knockout mouse urine metabolomics data. For 6-month-old animals, n=4. For 1-month-old animals, n=3 control and n=4 for knockout animals. Proteomics data: Urine proteomics raw counts data for 1 and 6-month-old Ren1cKO and control mice, related to Figures 4 and 5. Raw spectral counts for control and knockout mouse urine metabolomics data. For 6-month-old animals, n=4. For 1-month-old animals, n=3 control and n=4 for knockout animals.