Effect of treatment with tempol or L-NAME on ABR threshold shift and the decrease in Cx26 level in noise-exposed mice.

Mice were exposed to noise at 110 dB SPL for 1 h. At 30 min before the onset of noise exposure, the animals were given tempol (30 mg/kg, i.p.) or L-NAME (1 mg/kg, i.p.). The ABR threshold was assessed at the frequencies of 4, 12, and 20 kHz on immediately and day 7 post-noise exposure. Values are the mean ± S.E. from 3–12 separate animals per each group. For determination of the Cx26 level by immunoblot analysis, tissue lysates were prepared from the SL at 4 h post-noise exposure. Values are the mean ± S.E. from 4 separate animals per each group.
**P<0.01, significantly different from control value obtained for naïve animals.
#P<0.05,
##P<0.01, significantly different from the value obtained for noise-exposed animals without drug treatment.