Longitudinal three-photon imaging for tracking amyloid plaques and vascular degeneration in a mouse model of Alzheimer’s disease

Vascular abnormalities may contribute to amyloid-beta accumulation and neurotoxicity in Alzheimer’s disease (AD). Monitoring vascular degeneration as AD progresses is essential. Three-photon fluorescence microscopy (3PM) enables high-resolution deep tissue imaging with minimal invasiveness and photodamage. We demonstrated the potential of three-photon imaging to track vascular and amyloid-related changes in deep cortical structures. It offers a tool for studying the interplay between vascular and amyloid pathologies in AD, supporting future research into disease mechanisms and therapeutic strategies. This dataset contains all three-photon imaging data used in the manuscript, including plaques and vascular structures across multiple mice, fields of view (FOVs), and two longitudinal imaging sessions. GFP labeled neurons with vessels as well as neonatal lung imaging for power calibration can also be found in the folder.