Inflammatory response and metabolismrelated processes were involved in transcriptomic changes following anti-ANGPTL3/IL22 administration

To better explore the possible mechanisms associated with the improvement of DN by anti-ANGPTL3/IL22, transcriptome sequencing was performed on kidney tissues.Total RNA was extracted from renal tissues and randomly fragmented into small fragments after enrichment by Oligo dT magnetic beads. Double-stranded cDNA was purified using AMPure XP beads and then modified with End Repair Mix to add poly A tails and sequencing connectors. Subsequently, it was converted to single-stranded DNA by USER enzyme, and PCR amplification was performed for 15 cycles to finally form a cDNA library. Finally, PCR clustering was performed on the cBot instrument, and sequencing was completed using the 2*151 mode.