NNMT promotes tubular senescence and fibrosis in chronic kidney disease (db/m and db/db mice kidney)

Diabetic kidney disease is the leading cause of chronic kidney disease. db/db mice, which carry a deletion mutation resulting in a dysfunctional leptin receptor, exhibit hyperphagia, obesity, and elevated insulin levels, making them a widely used preclinical model of diabetic kidney disease. At 16 weeks of age, db/db mice showed enriched transcriptomic signatures of cellular senescence in their kidneys compared to control db/m mice. Overall design: 2 groups of 8 (db/m) and 9 (db/db) animals. Kidney tissue from 16-week old male BKS.Cg-Dock7m +/+ Leprdb/J HE/HE (db/m) and BKS.Cg-Dock7m +/+ Leprdb/J WT/HO (db/db) mice.