Human subcutaneous preadipocytes RNAseq

we performed RNA-seq and pathways analysis on human preadipocytes isolated from abdominal (A) and gluteofemoral (GF) fat of 10 apple-shaped women and 7 pear-shaped women . Out of the 23,511 annotated transcripts, 636 and 752 genes were differentially expressed in apple- compared to pear-shaped cells in A-FAT and GF-FAT respectively. Interestingly, one of the most significantly over-represented pathways from the apple-specific gene list was “Nucleotide Excision Repair pathway”. Overall design: Culture preadipocytes isolated from Abdominal (A) and gluteofemoral (GF) adipose tissue obtained from premenopausal apple-shaped and pear-shaped women were used to perform transcriptome analysis by RNA-seq