Dataset 3: Influenza viruses induce cross-reactive antibody responses in vitro

B cells were obtained by negative selection, and then stimulated with CpG ODN 2006 or together with A/Vic11 for 6 days. Cross-reactive antibodies binding to H1, H2, H5, H6, H7 and B influenza subtypes in B cell culture were measured by mPlex-Flu assay. (A) Fold change in cross-reactive antibodies. All values of IgG levels (MFI) were subtracted those of medium before calculating fold change. Only those values of IgG induced by CpG plus A/Vic11 viruses, which were greater than 100, were selected to calculate fold change. Each symbol represents the median of fold change in levels of IgG induced by CpG plus H3 to IgG stimulated by CpG 2006 ODN alone. (B) Correlation between influenza specific antibodies and HA antigenic sequence.