The impact of the surfactant Break-Thru® S 301 on the recovery of springtail Folsomia candida juveniles in OECD 232 reproduction test by different extraction methods@en

The data orginate from lab experiments testing the impact of the surfactant Break-Thru® S 301 on the reproduction and other life history endpoints of the springtail Folsomia candida. The test organisms were exposed for 28 days in three different test soils (sandy, sandy-organic and loamy) and different extraction methods for assessing the number of reproduced juveniles, i.e. heat extraction and flotation. The comparison of these two methods could demonstrate that flotation as the standard extraction method, which is recommended in the most important test guidelines such as OECD 232, produces false-positive effects due to its surface activity in the flotation liquid. Furthermore, a high impact of soil type on the extent of this artefact was proven.本数据集源自实验室实验，旨在探究表面活性剂Break-Thru® S 301对弹尾纲生物等节跳虫（Folsomia candida）的繁殖及其他生活史终点的影响。试验生物在三种不同的试验土壤（砂土、砂质有机质土与壤土）中暴露28天，同时采用热提取法与浮选法两种不同的提取方案，以评估繁殖产生的幼体数量。通过对比这两种提取方法可知，作为多数核心试验指南（如OECD 232）推荐的标准提取方法，浮选法因在浮选液中具备表面活性，会产生假阳性效应。此外，研究证实土壤类型对该实验人为假象的严重程度具有显著影响。