High-throughput unbiased RNA-Sequencing (RNA-Seq) to profile gene expression in ipsilateral L4-L6 DRG obtained from rats of Sham+Veh, CPIP+Veh and CPIP+ETA groups

Since TNF-a is a pro-inflammatory cytokine that can promote neuroinflammation to mediate chronic pain, we then explored which signaling molecules or pathways in DRG were affected by TNF-a and contributed to neuroinflammation in CPIP condition. Overall design: Examination of 3 different groups mRNA profiles in DRG:Sham+Veh, CPIP+Veh and CPIP+ETA