Transcriptomic profiling of primary mediastinal liposarcoma

Primary mediastinal liposarcoma (MLPS) is an extremely rare tumor with unclear molecular biology. We conducted RNA sequencing in five cases of MLPS and compared gene expression between dedifferentiated liposarcoma (DDLPS) and well-differentiated liposarcoma (WDLPS). Enrichment analyses and immune cell deconvolution revealed that DDLPS showed features of dedifferentiation, cell cycle activation, Wnt signaling, and immune-cold status. This dataset provides the first transcriptomic landscape of primary MLPS and offers insight into potential therapeutic targets. Total RNA was extracted from formalin-fixed paraffin-embedded (FFPE) tumor samples from five patients with mediastinal liposarcoma. RNA-seq libraries were prepared and sequenced on an Illumina NovaSeq 6000 platform. Processed data includes transcript per million (TPM) values and raw count matrices for downstream transcriptomic and immunologic analyses. *************************************************************** Raw files for human/patient samples were not submitted to GEO due to concerns about submitting personally identifiable sequence data for open access. ***************************************************************