File S1 - Characterization of the Caenorhabditis elegans HIM-6/BLM Helicase: Unwinding Recombination Intermediates

Figures S1 & S2. Figures S1A and S1B. Structures of DNA Substrates for Helicase Assays. The labeled oligonucleotides were annealed to unlabeled complementary strands as described in Materials and Methods. Figure S2A. A Sequence alignment between human, murine, and C. elegans BLM holomogs. A domain containing Walker A-type (GXGGKS) is conserved. Nucleotide sequences (aaa) for lysine residue (275) of HIM-6 was mutated to nucleotide sequences (gcg) for alanine residue. Figure S2B. ATPase activity of HIM-6 (K275A) mutant. Reaction mixtures contained HIM-6 (K275A), 2 mM ATP, and 250 ng/μl DNA effector and were incubated at 37°C for 30 min. The amount of inorganic phosphate (Pi) released by ATP hydrolysis was determined as described in the Experimental procedures. X, no DNA; ○, circular M13mp18 ssDNA. (PDF)